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Amsphere™ A3

Amsphere™ A3 is a next generation Protein A chromatography resin for advanced protein separation in downstream processing of therapeutic antibodies manufacturing.

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How Can Amsphere™ A3 Help Me?

Besides an outstanding high capacity, Amsphere™ A3 has an overall improved process robustness, flow characteristics, optimized impurity removal, productivity and resin lifetime.



Protein A Ligand
High DBC via controlled conformation and orientation
High alkaline stability from protein engineering
Surface modification
Low HCP levels by surface hydrophilization
Base bead formulation
High DBC at high flow rate
Good pressure and flow properties via rigid crosslinking


Product Lineup

  • North America & EMEA Regions



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  • APAC Region




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How do I pack Protein A resin in a column?

This video explains how to pack a column with our Amsphere™ A3 Protein A resin. It explains the small scale packing in an 1.6cm Diameter Column (HiScale™2 16/40).

2 HiScale™ is a trademark of GE Healthcare.


Why use AMSPHERE™ A3?

  • Outstanding Dynamic Binding Capacity

    A highly cross-linked bead structure and controlled attachment of the protein A ligand results in a superior dynamic binding capacity.
    At higher flow rates the semi-rigid bead structure allows to achieve up to 50% higher DBC compared to the market standard product at low to moderate residence times of 2-6 minutes.

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  • Impurity Clearance At Industry Standard

    The surface treatment of the polymeric base matrix resulted in low HCP, DNA, virus and aggregate levels that are very similar to hydrophilic backbones such as agarose.



  • Superior Caustic Stability

    Amsphere™ A3 can resist up to 300 cycles with 0.1 M sodium hydroxide as CIP solution (15 minutes of contact time) while maintaining a dynamic binding capacity above 90%. In addition, 0.5 M sodium hydroxide can be used for at least 100 cycles (15 minutes of contact time).



  • Regulatory Compliance

    The manufacturing process of Amsphere™ A3 complies with all regulations that enable the usage of the final product for GMP compliant drug manufacturing. All raw materials including the polymeric beads and protein A ligand are manufactured within ISO certified manufacturing facilities owned by JSR.

    The entire production process is free from animal-derived components and is certified BSE-TSE Free.

    The Regulatory Support File is available under a Confidential Disclosure Agreement (CDA).

  • Pre-Packed Formats & Validated Protein A Leaching Kit

    Ready-to-use pre-packed RoboColumn®s (200 – 600 µL) and small scale 1 mL and 5 mL columns are available for screening purposes and process development. Larger formats including GMP compliant pre-packed columns are also available on request.

    An Amsphere™ A3 ligand specific ELISA kit is available through Cygnus Technologies. Please click here to order the kit.


Universal Affinity Resin for
Non-classical mAbs

Simplify Your Supply Chain
& Minimize Costs

Impurity Clearance

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Tutorial: Packing of Protein-A resins in
lab-, pilot- & large-scale columns; how to successfully pack the first time

This web-based, technical seminar helps scientists, engineers, and operators successfully pack Amsphere A3 Protein-A resin for lab-, pilot- and large-scale columns. Each column scale is covered separately, and best practices are shared.


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