Amsphere™ A3 is a next generation Protein A chromatography resin for advanced protein separation in downstream processing of therapeutic antibodies manufacturing.

Current A3 Programs
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Commercial Programs 1
How Can Amsphere™ A3 Help Me?
Besides an outstanding high capacity, Amsphere™ A3 has an overall improved process robustness, flow characteristics, optimized impurity removal, productivity and resin lifetime.
Protein A Ligand High DBC via controlled conformation and orientation High alkaline stability from protein engineering |
Surface modification Low HCP levels by surface hydrophilization |
Base bead formulation High DBC at high flow rate Good pressure and flow properties via rigid crosslinking |
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Catalog Numbers & Product Sizes
Why use Amsphere™ A3?
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Outstanding Dynamic Binding Capacity
A highly cross-linked bead structure and controlled attachment of the protein A ligand results in a superior dynamic binding capacity.
At higher flow rates the semi-rigid bead structure allows to achieve up to 50% higher DBC compared to the market standard product at low to moderate residence times of 2-6 minutes. -
Impurity Clearance At Industry Standard
The surface treatment of the polymeric base matrix resulted in low HCP, DNA, virus and aggregate levels that are very similar to hydrophilic backbones such as agarose.
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Superior Caustic Stability
Amsphere™ A3 can resist up to 300 cycles with 0.1 M sodium hydroxide as CIP solution (15 minutes of contact time) while maintaining a dynamic binding capacity above 90%. In addition, 0.5 M sodium hydroxide can be used for at least 100 cycles (15 minutes of contact time).
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Regulatory Compliance
- Amsphere™ A3 resin manufacturing, testing, packaging, storage, and distribution are all done in accordance with ISO9001 standards and certified.
- All raw materials used in the manufacturing process of Amsphere™ A3 are evaluated and free of animal-derived substances and are considered safe regarding BSE/TSE risk.
- Amsphere™ A3 resin is used in the biopharma industry for the GMP manufacturing process of clinical and commercially approved biologics.
- The Regulatory Support File is available under a Confidential Disclosure Agreement (CDA).
- Amsphere™ A3 resin manufacturing, testing, packaging, storage, and distribution are all done in accordance with ISO9001 standards and certified.
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Pre-Packed Formats & Validated Protein A Leaching Kit
Ready-to-use pre-packed RoboColumn®s (200 – 600 µL) and small scale 1 mL and 5 mL columns are available for screening purposes and process development. Larger formats including GMP compliant pre-packed columns are also available on request.
An Amsphere™ A3 ligand specific ELISA kit is available through Cygnus Technologies. Please click here to order the kit.
Why Amsphere A3 is an Outstanding Choice for Antibody Fragment & FC-Fusion Purification
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Impurity Clearance
On-Demand Webinar
Development of Protein A Continuous Chromatography
We are excited to have a speaker from our affiliate company, KBI Biopharma, a global CDMO, present on the development of Protein A continuous Chromatography.
Multicolumn continuous chromatography (MCC) is an emerging technology that can significantly reduce material costs and time in plant. This mode of chromatography can lead to shorter processing times and improve efficiency as well as productivity compared to a traditional batch process. Implementation of a fully integrated end-to-end continuous process has been limited due to the complexity and scalability of the process.
This presentation will focus on the conversion of a batch ProA step using Amsphere A3 resin to a 4-column continuous process on a BioSMB system. We evaluated the number of columns and resin volume needed to achieve balanced savings between resin/buffer usage and processing time when scaling up the process across a range of feed titers.
Presenter: Geok-Yong Yow Ph.D.
Scientist, KBI Biopharma Inc.