Why Amsphere A3 is an Outstanding Choice for Antibody Fragment & Fc-Fusion Purification

Universal Affinity Resin for Non-classical mAbs

Amsphere A3 has the ability to bind Fc-fusion proteins and VHH or VH-based antibody fragments such as single domain antibodies (sdAb) at a DBC higher than the capacities of other protein A resins.

VHH sdAbs - 110 molecules were screened, of which 99% showed binding to A3 ligand. (Collaboration with Ablynx NV)

DBC of Fc-Fusions for A3 vs. Competitor Media

Simplify Your Supply Chain
& Minimize Costs

With Amsphere A3, purification of mAbs, antibody fragments and Fc-fusion molecules can be achieved with a single resin platform. 

HCP Levels in Fc-Fusion Eluate Pools for A3 vs. Competitor Media

Excellent Impurity Clearance

Amsphere A3 meets industry standard for impurity clearance in Fc-fusion and antibody fragment processes. A3 performs just as well as other competitor media for clearance of host cell proteins in Fc-fusion protein processes. Additionally, a host cell protein clearance of 2.7 LRV was achieved with a VHH based antibody fragment process.

Download Whitepaper

Purification of antibody fragments and single domain antibodies with Amsphere™ A3 Protein A resin

The wide range of antibody fragment variants for which Amsphere A3 can be used as a capture resin is described, together with insight to the related
binding mechanisms obtained via x-ray crystallography.

WEBINAR: PROTEIN A RESIN FOR ANTIBODY FRAGMENT PURIFICATION

ON DEMAND

Get a Quote

We're ready to help you with your antibody fragment or Fc-fusion purification process.